Journal: Frontiers in Immunology
Article Title: In silico and in vitro arboviral MHC class I-restricted-epitope signatures reveal immunodominance and poor overlapping patterns
doi: 10.3389/fimmu.2022.1035515
Figure Lengend Snippet: Panoramic Evaluation of HLA-A*02:01 Reactivity for Peptides Derived from Arbovirus. Peptide microarray was performed with an HLA-A*02:01 dimeric protein in three concentrations (1µg/mL, 10µg/mL and 30µg/mL). Overall fluorescence of peptide-HLA-A*02:01 reactivity (top panels), absolute number of HLA-*02:01-reactive peptides (middle panels) and mean of fluorescence intensity of HLA-*02:01-reactive peptides (lower panels) were evaluated. Chi-square test (χ2) was used to compare the number of HLA-A*02:01-reactive. Obtained p values are indicated above each bar. Comparisons regarding the mean of fluorescence intensity of HLA*02:01-reactive peptides were performed using the Kruskal-Wallis. Statistical significances were considered for p < 0.05 and are indicated by connecting lines.
Article Snippet: Evaluation of the HLA-A*02-restricted immunodominant peptide repertoire was performed using PEPperCHIP © (PEPperPRINT © , Heidelberg, Germany) custom peptide microarray ( ).
Techniques: Derivative Assay, Peptide Microarray, Fluorescence